Volume: 46 Issue: 3
Year: 2026, Page: 260-266, Doi: https://doi.org/10.51248/v46i3.62
Received: June 5, 2026 Accepted: June 26, 2026 Published: July 2, 2026
Background: Primary dysmenorrhea (PD) is widely prevalent gynaecological disorder that substantially impairs health-related quality of life (HRQoL). While studies have demonstrated a relationship between Vitamin D deficiency and heightened menstrual pain, there is limited evidence regarding the influence of Vitamin D3 supplementation on multiple domains of HRQoL in women with dysmenorrhea.
Objectives: To assess effect of Vitamin D3 supplementation on HRQoL in women presenting with primary dysmenorrhea and concurrent Vitamin D deficiency.
Materials and Methods: This study presents secondary analysis of HRQoL data from prospective interventional clinical trial registered with Clinical Trials Registry- India. A total of 65 women between 14 and 40 years of age, diagnosed with primary dysmenorrhea and having serum Vitamin D levels below 30 ng/mL, were administered oral Vitamin D3 at 60,000 IU weekly over eight-week period. HRQoL was evaluated by employing Short Form-36 (SF-36) questionnaire at baseline, four weeks, and eight weeks. Secondary outcome measures included pain intensity via Visual Analogue Scale (VAS), menstrual blood loss via Pictorial Blood Loss Assessment Chart (PBLAC), and safety monitoring throughout study period.
Results: Significant improvements were documented across all eight SF-36 domains at both four-week and eight-week assessments relative to baseline (P < 0.001). Mean serum Vitamin D levels rose from 19.13 ± 5.86 ng/mL to 41.65 ± 8.85 ng/mL following supplementation (P < 0.001). VAS pain scores declined markedly, whereas menstrual blood loss measured by PBLAC did not change significantly.
Conclusion: Vitamin D3 supplementation significantly improved HRQoL in women with primary dysmenorrhea and Vitamin D deficiency, with benefits extending beyond pain reduction alone.
Keywords: Primary dysmenorrhea; Vitamin D deficiency; Health-related quality of life; Vitamin D3; SF-36.
1. Fenning NR. Dysmenorrhea. Curr Obstet Gynaecol. 2005;15:394-401.
2. Ju H, Jones M, Mishra G. The prevalence and risk factors of dysmenorrhea. Epidemiol Rev. 2014;36(1):104–13.
3. Omidvar S, Bakouei F, Amiri FN, Begum K. Primary Dysmenorrhea and Menstrual Symptoms in Indian Female Students: Prevalence, Impact and Management. Glob J Health Sci. 2016; 8(8):135–44.
4. Dawood MY. Primary dysmenorrhea: advances in pathogenesis and management. Obstet Gynecol. 2006;108(2):428-41.
5. Hailemeskel S, Demissie A, Assefa N. Primary dysmenorrhea magnitude, associated risk factors, and its effect on academic performance: evidence from female university students in Ethiopia. Int J Womens Health. 2016;8:489-96.
6. Schoep ME, Adang EMM, Maas JWM, De Bie B, Aarts JWM, Nieboer TE. Productivity loss due to menstruation-related symptoms: a nationwide cross-sectional survey among 32 748 women. BMJ Open. 2019;9(6):e026186.
7. Matsumura K, Tsuno K, Okamoto M, Takahashi A, Kurokawa A, Watanabe Y, et al., The association between the severity of dysmenorrhea and psychological distress of women working in central Tokyo-a preliminary study. Int J Environ Res Public Health. 2023;20(21):7021.
8. Iacovides S, Avidon I, Baker FC. What we know about primary dysmenorrhea today: a critical review. Hum Reprod Update. 2015;21(6):762-78.
9. Marjoribanks J, Ayeleke RO, Farquhar C, Proctor M. Nonsteroidal anti‐inflammatory drugs for dysmenorrhoea. Cochrane Database Syst Rev. 2015;10(7):146-50.
10. Chebet JJ, McMahon SA, Greenspan JA, Mosha IH, Callaghan-Koru JA, Killewo J, et al., Every method seems to have itsproblems- perspectives on side effects of hormonal contraceptives in Morogoro Region, Tanzania. BMC Womens Health. 2015;15(1):1–12.
11. Aparna P, Muthathal S, Nongkynrih B, Gupta SK. Vitamin D deficiency in India. J Family Med Prim Care. 2018;7(2):324-30.
12. Christakos S, Dhawan P, Verstuyf A, Verlinden L, Carmeliet G. Vitamin D: Metabolism, Molecular Mechanism of Action, and Pleiotropic Effects. Physiol Rev. 2016 Jan;96(1):365-408.
13. Anagnostis P, Karras S, Goulis DG. Vitamin D in human reproduction: a narrative review. Int J Clin Pract. 2013;67(3):225-35.
14. Hoffmann MR, Senior PA, Mager DR. Vitamin D supplementation and health-related quality of life: A systematic review of the literature. J Acad Nutr Diet. 2015;115(3):406–418.
15. Chen YC, Chiang YF, Lin YJ, Huang KC, Chen HY, Hamdy NM, et al., Effect of Vitamin D Supplementation on Primary Dysmenorrhea: A Systematic Review and Meta-Analysis of Randomized Clinical Trials. Nutrients. 2023;15(13):2830.
16. Kalra K, Goyal S, Chauhan MB. Evaluating the impact of Vitamin D deficiency on analgesic use in primary dysmenorrhoea: A correlation study. Indian J Physiol Pharmacol. doi: 10.25259/IJPP_488_2024
17. Holick MF, Binkley NC, Bischoff-Ferrari HA, Gordon CM, Hanley DA, Heaney RP, et al., Evaluation, treatment, and prevention of vitamin D deficiency: An Endocrine Society Clinical Practice Guideline. J Clin Endocrinol Metab. 2011;96(7):1911–1930.
18. Ware JE, Sherbourne CD. The MOS 36-item short-form health survey (SF-36). I. Conceptual framework and item selection. Med Care. 1992;30(6):473–83.
19. Larroy C. Comparing visual-analog and numeric scales for assessing menstrual pain. Behav Med. 2002;27(4):179-81.
20. El-Nashar SA, Shazly SA, Famuyide AO. Pictorial blood loss assessment chart for quantification of menstrual blood loss: a systematic review. Gynecol Surg. 2015;12:157-63.
21. Zarei S, Mohammad-Alizadeh-Charandabi S, Mirghafourvand M, Effati-Daryani F. Effects of calcium-Vitamin D and calcium-alone on pain intensity and menstrual blood loss in women with primary dysmenorrhea: a randomized controlled trial. Pain Med. 2017;18(1):3-13.
22. Amzajerdi A, Keshavarz M, Ghorbali E, Pezaro S, Sarvi F. The effect of vitamin D on the severity of dysmenorrhea and menstrual blood loss: a randomized clinical trial. BMC Womens Health. 2023;23(1):1-7.
23. Bahrami A, Avan A, Sadeghnia HR, Esmaeili H, Tayefi M, Ghasemi F, et al., High dose vitamin D supplementation can improve menstrual problems, dysmenorrhea, and premenstrual syndrome in adolescents. Gynecol Endocrinol. 2018;34(8):659-63.
24. Moini A, Ebrahimi T, Shirzad N, Hosseini R, Radfar M, Bandarian F, et al., The effect of Vitamin D on primary dysmenorrhea with Vitamin D deficiency: a randomized double-blind controlled clinical trial. Gynecol Endocrinol. 2016;32(6):502-5.
25. Rahnemaei FA, Gholamrezaei A, Afrakhteh M, Zayeri F, Vafa MR, Rashidi A, et al., Vitamin D supplementation for primary dysmenorrhea: a double-blind, randomized, placebo-controlled trial. Obstet Gynecol Sci. 2021;64(4):353-63.
Sarita Goyal, Kanchan Kalra, Meenakshi B Chauhan. Impact of Vitamin D3 Supplementation on Health-Related Quality of Life in Women with Primary Dysmenorrhea and Vitamin D Deficiency: A Secondary Analysis. Biomedicine: 2026, 46(3): 260-266. https://doi.org/10.51248/v46i3.62